Vimentin anticorps (AA 2-466)

N° du produit ABIN969454

Cet anticorps Souris Monoclonal détecte spécifiquement Vimentin dans WB, IHC et ELISA. Il présente une réactivité envers Humain et Singe et a été mentionné dans 2+ publications.

Aperçu rapide pour Vimentin anticorps (AA 2-466) (ABIN969454)

Antigène: Vimentin (VIM)

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 4F2E9

Détail du produit anti-Vimentin anticorps

Épitope: AA 2-466

Fonction: Vimentin Antibody

Purification: Ascitic fluid

Immunogène: Purified recombinant fragment of Vimentin (aa2-466) expressed in E. Coli.

Isotype: IgG1

Information d'application

Indications d'application: ELISA: 1/10000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Références pour anticorps anti-Vimentin (ABIN969454)

Yates, Zetterberg, Rajeev, Reiss, Rittling: "Promoter-independent regulation of vimentin expression in mammary epithelial cells by val(12)ras and TGFbeta." dans: Experimental cell research, Vol. 313, Issue 17, pp. 3718-28, (2007) (PubMed).

Gilles, Polette, Mestdagt, Nawrocki-Raby, Ruggeri, Birembaut, Foidart: "Transactivation of vimentin by beta-catenin in human breast cancer cells." dans: Cancer research, Vol. 63, Issue 10, pp. 2658-64, (2003) (PubMed).

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Sujet: Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Poids moléculaire: 54 kDa

ID gène: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis